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Friday, 10 October 2014

Isolation of magnetotactic bacteria and its analysis of magnetosomes

Introduction
Method
Results
Conclusion

References
These bacteria occur in deeper region:-
Many MTB are able to survive only in environments with very limited oxygen, and some can exist only in completely anaerobic environments Magnetotactic bacteria are found in the sediments of many aquatic environments .
§These are Gram-negative members of the Domain Bacteria and belong phylogenetically to various subgroups of the Proteobacteria.
§ They  are motile by means of flagella
§Magnetotactic bacteria produce their magnetic particles in chains
§The most abundant type of MTB occurring in        environmental samples, especially sediments, are coccoid cells possessing two flagellar bundles on a some what flattened side. This "bilophotrichous" type of flagellation gave rise to the tentative .
§They are ubiquitous in aquatic habitats and cosmopolitan in distribution. Locally confined to or slightly below the oxic-anoxic interface.
MTB orient and migrate along geomagnetic field lines –  this ability is based on intracellular magnetic structures known as magnetosomes. They contain 15 to 20 magnetite crystals that together act like compass needle to orient magnetotactic bacteria in geomagnetic fields.
Each magnetite crystal with in a magnetosome is surrounded by a lipid bilayer ,and specific soluble and transmembrane proteins are sorted to the membrane.
Overall, magnetosome crystals have high chemical purity, narrow size ranges, species-specific crystal morphologies and exhibit specific arrangements with in the cell. These features indicate that the formation of magnetosomes is under precise biological control and is mediated biomineralization
Invagination of the cytoplasmic membrane and vesicle formation for the magnetosome membrane precursor
Accumulation of ferrous/ferric ions in the cell and the vesicles
Strictly controlled iron oxidation–reduction 

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